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Wednesday, April 29, 2020 | History

5 edition of In situ detection of DNA damage found in the catalog.

In situ detection of DNA damage

methods and protocols

by

  • 393 Want to read
  • 25 Currently reading

Published by Humana Press in Totowa, N.J .
Written in English

    Subjects:
  • DNA damage -- Research -- Methodology

  • Edition Notes

    Includes bibliographical references and index

    Statementedited by Vladimir V. Didenko
    SeriesMethods in molecular biology -- v. 203, Methods in molecular biology (Clifton, N.J.) -- 203
    ContributionsDidenko, Vladimir V
    Classifications
    LC ClassificationsQH465.A1 I496 2002
    The Physical Object
    Paginationxvii, 313 p., [4] p. of plates :
    Number of Pages313
    ID Numbers
    Open LibraryOL17044741M
    ISBN 100896039528
    LC Control Number2002017245


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In situ detection of DNA damage Download PDF EPUB FB2

In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can.

In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: In situ detection of DNA damage book and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism.

In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can Format: Hardcover.

In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa).

In Situ Detection of DNA Damage; find Sigma-Aldrich-I MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. It allows the possibility to compare the level of DNA damage and the effectiveness of its repair in certain regions of the genome.

Comet-FISH is a combination of two well-known methods: the Comet assay (single-cell gel electrophoresis) and fluorescence hybridization in situ. It is a sensitive and rapid method for the detection of DNA by: 1.

In situ detection of DNA damage: methods and protocols. Labeling DNA breaks using DNA polymerase I or its Klenow fragment -- DNA damage detection using DNA polymersase I or its Klenow fragment: applicability, specificity, limitations \/ Jan Hein van Dierendonck -- Labeling DNA breaks in situ by Klenow enzyme \/ Katherine A.

Wood. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism.

Chapters present all major assays used in molecular Format: Hardcover. Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and Price: $ Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others.

Individual cells within the same tissue or in cell culture may. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells.\/span>\"@ en.

Chapter 10 Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair Altmetric Badge Chapter 11 Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH).

DNA damage detection In situ, ex vivo and In vivo - Methods and protocols Article (PDF Available) in European journal of histochemistry: EJH 55(2):5 April with Reads How we measure 'reads'Author: Carlo Alberto Redi. In "In Situ Detection of DNA Damage: Methods and Protocols", Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa).

Request PDF | Detection of DNA Damage by Comet Fluorescence In Situ Hybridization | INTRODUCTION The Comet-FISH technique is a useful tool for. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism.

Chapters present all major assays used in molecular. DNA damage is a ubiquitous phenomenon experienced by microbes in the environment. Potential DNA-damaging agents include enzymes (e.g., restriction enzymes encoded by addiction modules or bacteriophage) (1, 23, 31), as well as nonenzymatic attacks via physical (e.g., irradiation) and chemical (e.g., secondary metabolites such as antibiotics and reactive oxygen species [ROS]) agents ().Cited by: We here describe a technique termed STRIDE (SensiTive Recognition of Individual DNA Ends), which enables highly sensitive, specific, direct in situ detection of single- or double-strand DNA breaks (sSTRIDE or dSTRIDE), in nuclei of single cells, using fluorescence microscopy.

The sensitivity of STRIDE was tested using a specially developed CRISPR/Cas9 DNA damage induction system. Kup książkę In Situ Detection of DNA Damage (Vladimir V.

Didenko) za jedyne zł u sprzedawcy godnego zaufania. Zajrzyj do środka, czytaj recenzje innych czytelników, pozwól nam polecić Ci podobne tytuły z naszej ponad milionowej kolekcji.

In: Didenko V. (eds) DNA Damage Detection In Situ, Ex Vivo, and In Vivo. Methods in Molecular Biology (Methods and Protocols), vol Humana Press, Totowa, NJCited by: Electrochemical voltammetric in situ detection of dsDNA oxidative damage caused by reduced adriamycin, an antibiotic of the family of anthracyclines, intercalated into DNA, was carried out using a DNA-biosensor.

Oxidation and reduction of adriamycin molecules intercalated in dsDNA were investigated in order to understand the in vivo mechanism Cited by: Interestingly, this resulted in DNA fragmenting into approximately – bases that separated on an agarose gel in the form of a ladder.

Therefore, this assay was called the “DNA laddering” assay and by far is the most common assay used to determine apoptosis in cultured cells as well as in tissues. In Situ Detection of DNA Strand Breaks. Coats P. J., Save V., Ansari B., and Hall P.

() Demonstration of DNA damage/repair in individual cells using in situ end labeling: association of p53 with sites of DNA damage. Cited by: 2. The EpiQuik™ In Situ DNA Damage Assay Kit is a convenient set of tools that allows the experimenter to detect DNA damage or apoptosis by measuring phosphorylation of H2AX Ser in kit is ready-to-use and provides all the essential components needed for specifically measuring DNA damage in situ through phospho H2AX Ser detection using cultured adherent cells.

An important function of many checkpoints is to assess DNA damage, which is detected by sensor mechanisms. When damage is found, the checkpoint uses a signal mechanism either to stall the cell cycle until repairs are made or, if repairs cannot be made, to target the cell for destruction via apoptosis (effector mechanism).

Introduction. Cancer is the second most frequent cause of death worldwide according to the World Health Organisation (WHO) ().One of the most aggressive forms of Non-Hodgkin’s lymphoma, diffuse large B-cell lymphoma (DLBCL), is diagnosed in more than 50% of lymphoma patients over the age of 65 ().As for most malignant diseases, the risk of developing DLBCL increases with age, and more than Author: Madeleine Ruhe, Dominik Rabe, Christoph Jurischka, Julia Schröder, Peter Schierack, P.

Markus Decker. The DNA Damage Quantification Kit utilizes the ARP (AldehydeReactive Probe) reagent that reacts specifically with an aldehyde group which is the openring form of the AP sites. After treating DNA containing AP sites with ARP reagents, APsites are tagged with biotin residues, which can be quantified using avidin-biotin assayfollowed by a Ratings: 7.

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence was developed by biomedical researchers in the early s to detect and localize the presence or absence of specific DNA sequences on chromosomes.

The DNA-electrochemical biosensor is a very good model for evaluation of nucleic acid damage, and electrochemical detection a particularly sensitive and selective method for the investigation of specific interactions. The electrochemical sensor for detecting DNA damage consists of a glassy carbon electrode with DNA immobilized on its by: BACKGROUND.

In situ hybridization is a technique that is used for localization and detection of specific DNA and RNA sequences in cells, preserved tissue sections, or entire tissue (whole mount in situ hybridization, Fig.

1) by hybridizing the complementary strand of a nucleotide probe to a particular hybrids can be visualized by autoradiography for probes labeled radioactively Cited by: @article{osti_, title = {Detection of DNA damage in individual cells by flow cytometric analysis using anti-DNA monoclonal antibody}, author = {Frankfurt, O.S.}, abstractNote = {A new method for the measurement of DNA damage in individual cells treated with alkylating agents is described.

The method is based on the binding of anti-DNA monoclonal antibody to DNA in situ. Book Author: Immunohistochemistry.

Essential Elements and Beyond. Springer, Book Chapters: Hopp N, Hagen J, Aggeler B and A Kalyuzhny () Express γ-H2AX Immunocytochemical Detection of DNA Damage. In: Fast Detection of DNA Damage. Methods Mol Biol. Vol DNA Damage Detection In Situ, Ex Vivo, and In Vivo Autor Vladimir V.

Didenko. Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. The genome integrity of living organisms is constantly threatened by endogenous cellular metabolic processes and environmental agents.

To quantify these low, physiologically relevant levels of DNA damage, a single-cell gel electrophoresis (comet) combined with strand-specific fluorescence in situ hybridization (FISH)-based approach has been by: 3. In this review, we will summarize and discuss the use of in situ procedures to detect the formation of DNA-protein complexes after radiation-induced DNA damage.

This type of analysis provides important information on the spatial localization and temporal resolution of the formation of such complexes, at the single-cell level, allowing the study Author: Giulio Ticli, Ennio Prosperi.

The HCS DNA Damage kit simultaneously and quantitatively measures two important cell-health parameters: DNA damage and cytotoxicity. DNA damage is detected using an antibody against phosphorylated H2AX (Ser) which is induced in response. Types. Damage to DNA that occurs naturally can result from metabolic or hydrolytic processes.

Metabolism releases compounds that damage DNA including reactive oxygen species, reactive nitrogen species, reactive carbonyl species, lipid peroxidation products and alkylating agents, among others, while hydrolysis cleaves chemical bonds in DNA. Naturally occurring oxidative DNA damages arise at.

Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR Nadja Patenge; Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt Ended DNA Breaks by In Situ Ligation Vladimir V. Didenko; Part III: ACCELERATED DETECTION (time-saving versions of conventional techniques) The EpiQuik™ 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically detect and quantify oxidative DNA damage (8-OHdG) directly using DNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses in a variety of forms including, but not limited to, cultured cells, fresh and frozen tissues.

Damage from spur and blob energy depositions can lead to multiple close sites of DNA damage, termed locally multiply damaged site Frequency of DNA damage from photon radiation A dose that induces on average one lethal event per cell is the D 0 dose; statistically ~37% of cells in a population exposed to D 0 dose will survive, while other cells Base damage: >/cell.

@article{osti_, title = {DNA DAMAGE QUANTITATION BY ALKALINE GEL ELECTROPHORESIS.}, author = {SUTHERLAND, B M and BENNETT, P V and SUTHERLAND, J C}, abstractNote = {Physical and chemical agents in the environment, those used in clinical applications, or encountered during recreational exposures to sunlight, induce damages in DNA.

Understanding the .Damage to DNA, including oxidative DNA damage, has been implicated in the development of a variety of cancers including colon, breast and prostate. Mutations to checkpoint kinases can ultimately lead to decreased DNA repair and increased susceptibility to cancer.

Recently oxidative RNA damage has been described in conjunction with a wide variety of neurological diseases including Alzheimer’s.

This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis.

It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each Book Edition: 1.